KMID : 0364820160520020183
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Korean Journal of Microbiology 2016 Volume.52 No. 2 p.183 ~ p.191
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Isolation and characterization of anaerobic microbes from marine environments in Korea
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Kim Won-Duck
Lee Jung-Hyun Kwon Kae-Kyoung
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Abstract
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Marine bacteria have represented unique physiologies and products which are not discovered from terrestrial organisms.
There has been great interest to utilize and develop marine bacteria in many industrial sectors. Recently, we isolated and
characterized anaerobic bacteria from various marine environments in Korea to search organic acids fermenting strains. From our enrichment performed under anaerobic condition, 65 strains were isolated and identified by the 16S rRNA gene sequence analysis. Among them, eleven strains were selected for phylogenetical and biochemical analysis. All tested strains were affiliated with Class Clostridia except one with Class Bacteroidia. Most of strains produce acetate (6 strains) with butyrate (2 strains) and/or formate (4strains). Strain MCWD5 transformed 40% of glucose to extracellular polymeric substances. These results indicate that many novel anaerobic microorganisms which have great potential in commercial application are distributed in the marine environments ofKorean Peninsula.
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KEYWORD
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anaerobic marine bacteria, Clostridia, enrichment culture, organic acid production
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